首页> 外文OA文献 >TATA box-binding protein (TBP) is a constituent of the polymerase I-specific transcription initiation factor TIF-IB (SL1) bound to the rRNA promoter and shows differential sensitivity to TBP-directed reagents in polymerase I, II, and III transcription factors.
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TATA box-binding protein (TBP) is a constituent of the polymerase I-specific transcription initiation factor TIF-IB (SL1) bound to the rRNA promoter and shows differential sensitivity to TBP-directed reagents in polymerase I, II, and III transcription factors.

机译:TATA盒结合蛋白(TBP)是与rRNA启动子结合的聚合酶I特异性转录起始因子TIF-IB(SL1)的组成部分,在聚合酶I,II和III转录因子中对TBP定向试剂表现出不同的敏感性。

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摘要

The role of the Acanthamoeba castellanii TATA-binding protein (TBP) in transcription was examined. Specific antibodies against the nonconserved N-terminal domain of TBP were used to verify the presence of TBP in the fundamental transcription initiation factor for RNA polymerase I, TIF-IB, and to demonstrate that TBP is part of the committed initiation complex on the rRNA promoter. The same antibodies inhibit transcription in all three polymerase systems, but they do so differentially. Oligonucleotide competitors were used to evaluate the accessibility of the TATA-binding site in TIF-IB, TFIID, and TFIIIB. The results suggest that insertion of TBP into the polymerase II and III factors is more similar than insertion into the polymerase I factor.
机译:检查了棘阿米巴castellanii TATA结合蛋白(TBP)在转录中的作用。针对TBP非保守N末端结构域的特异性抗体用于验证RNA聚合酶I TIF-IB的基本转录起始因子中TBP的存在,并证明TBP是rRNA启动子上定型起始复合物的一部分。相同的抗体在所有三个聚合酶系统中均抑制转录,但它们的作用不同。寡核苷酸竞争者用于评估TIF-IB,TFIID和TFIIIB中TATA结合位点的可及性。结果表明,将TBP插入聚合酶II和III因子比插入聚合酶I因子更相似。

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